0000006166 00000 n 0000001381 00000 n %PDF-1.5 %���� |��_���W�+�z ��^/���K�AO�=�D�AO�=�$�'= � � � � � � ��=�'�'�'�G�Q�QYSQSYSQSYSQS�Q�Q��M@���������w�������ٕ�����!�޿9d���=�3�3�3�3�3�3�3�3�3�3�3�3�3�3���} Smaller Volume Filter to sterilize, and store in the dark. h�b```b``�c`e`�� ̀ �@16�GA3�Hp�o�`NcH0q`�m``��ۻuuT$2Pd�K�`��2'�Lb�84�|��Fش2�؏l�,9ZyU���f�'N=,���1�qBȡ:�yS��b&�U1�y��h �Y�zP �C�R~�B�ҥ1�l�V%v15(�`s�r����+��d`0q�q�8@_��LJJJ��P� H�\�ˊ�0E�� 0000005617 00000 n }���9�|>��k�y;ݞ���nCrż_��t�:�UwJY��k��7�VY�����ñ~�Ƭ\~��U�_V��ګ�ż��t������/�8_l�7�[�-4}l1M[�ҳ�G}��^B�����B-�����J f�#49=8=9=8��zmZ�+�� Store the buffer at 4°C for several days. Adjust solution to desired pH using NaOH (typical pH … High Resolution }2�N�Fͷ��M��k�_g�R�y;}�����hb6ʪ�/�����j2�:c��Qq'�0�*ː��{5Y ~^�&/��N[�7j�ֶT�{�B��p��2��V�ܪa��Z U�v)��e-w��67���odLli�c48�Y�i��{�~��?ڢ�|��YY�+�f�I�4�~`TfsK���l �v��] "|������5ɇMn�ԃr)�Βq�ޒrk�r��@��zI> �Ag����n�:�-W���� C�hz�;|�'y4�t.�ɯ��x3m��F�����d�����������7�j ����=��A�M�٪�j���8�Op6� �c��&nO9�{��~�6�ù����>��֌]�pu��}�x�(^� �d�����^���]YU#��xDk�C����d *C��0 T�d �7Jb�܇>����2��Xٙ5�>D][� -��*Uu �,��d�[�&�q܋�����n��#l��.���~�����?ƾ>�ɝۮ���N��vYY����Go~����i��~��uߝ�l�t����6�w��n�S|���cǶ�����ó����7^c7�­V���T��q�v�F���^�Mz�N��4��_�!�j��&ކc�cw�ٲH��-�ҵ�b������J~/�_��k���;0�LMbΗ�l�9��\�$���\�=ك�,`�y�y%����t�p�t�p�t�p����:����A� p:����A� p:dCހ�� 7an�܄� rz 0000030420 00000 n 6x DNA loading buffer: For 100 mL • Weigh 60 g glycerol into a 100-mL graduated cylinder • Add 12 mL 0.5 M EDTA, pH 8 • Add 10 mg bromophenol blue • Bring up the volume to 100 mL with ddH 2O 10x DNA loading buffer: For 100 mL • Measure 20 mL 50x TAE into a 100-mL graduated cylinder • Add 40 g sucrose • Add 10 mg bromophenol blue It can be used as the electrolyte system component in isoelectric focusing electrophoresis (IEF) of two-dimensional gel electrophoresis. 0000004280 00000 n Recipe for 10x MOPS buffer, 1 L. 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g) 3.7 g EDTA, disodium dihydrate; MW 372.24 g/mol (again check MW of the salt you stock) add 800 ml of nuclease free distilled water; mix to dissolve. Adjust the pH to 7.5 with NaOH and then add DEPC-treated H2O to give a final volume of 1 L. Sterilize the buffer using a 0.22-μm filter. Adjust the volume of the solution to 1 liter with Diethyl pyrocarbonate (DEPC)-treated H2O. Add 3.72 g of Na 2 EDTA to the solution. Dissolve the reagents listed above in 50 mL of H2O. Change ), You are commenting using your Google account. 0000000956 00000 n 0000000016 00000 n Reconstitute with 1000 ml H2O to make 1X running buffer per bag of powder. ( Log Out /  a��5�Z� �_��9*(�� $I g����\d�A��@�l�l^LV� ��/��~�x5[m��ŏӏ ��0E�SX�Ա���Ԇ# G^��N���UjC���n�!�M0���$�]')���ih;M�~K��E���^2��1�Z�(Z6�M�5 oVܩ��E�E��T��t[�*SvNSr���tG]�*c[�Y���{�l������Z%s'=��U;H+��j���!9��;p�Jɸ乿���a��p����l-5/([� Bring the pH to 7.0 with NaOH (or if the MOPS sodium salt is used, then use glacial acetic acid to adjust the pH). Long Shelf Life MOPS, 3-(N-Morpholino)propanesulfonic acid, is a biological buffer, the buffer range is 6.5~7.9, which is suitable for the study of electron transfer and phosphorylation in chloroplast thin layer; it… H�tVMr55ܿS�b,[����8B Make fresh. Its chemical structure contains a morpholine ring. Useful pH Range: 6.5 - 7.9: Working concentration range: 20mM-1M 7, 8 0000008733 00000 n Add 4.1 g of Sodium Acetate to the solution. Change ), You are commenting using your Facebook account. 0000029925 00000 n ��:%��#F:�ĝ��?dJ��l1��i���~3?c��+�޹P���7P��v�����I�����>ZO���:GO����~�/rqy�>"g�S��Ǜ�{�0���o��1���?ob�6��!6E��^��_lJ��Mt:'y����q�;��K����N1ڶ.�W�������94ϐ�hN���F)P70���`C�'6`w�6�AY~�c0�:E-6"��:W�5[c^3�N*X� 8�(aoT��*T(*�� Tris-MOPS-SDS Running Buffer Powder are used for ExpressPLUS and SurePAGE gel transfer. s�-�3�3�3�3�3�3�-�-�-�-�-�M�v�ڕ55�55�55�kW�]����������s}�}�s���+sP��A��2E�س�A�9s0�`�7�� �F�����o�7�� �F�����o�7�� �ԩ� It is a structural analog to MES. 0000004243 00000 n The solution will turn yellow with time, but this is normal. Recipe for 10X buffer stock: Tris base 29 g Glycine 144 g Deionized water to 1,000 mL MOPS SDS running buffer: 50 mM MOPS, 50 mM Tris base, 0.1% SDS, 1 mM EDTA, pH 7.7 Recipe for 20X buffer stock: MOPS 104.6 g Tris base 60.6 g SDS 10 g EDTA 3.0 g Deionized water to 500 mL 0000016763 00000 n In biochemical experiments, the buffer recipe is often the top priority of the experiment, and a truly reliable recipe can definitely change the fate of the experiment.

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